Animal House
The Animal House Facility maintains thirty one strains of mice (inbred, outbred, transgenic, knock-out & immune deficient ) , three strains of rats, two strains of hamsters, three strains of rabbits and two strains of guinea pigs in different rooms. The average number of animals are maintained around 10,000 to 12000. The animal rooms are maintained at 22.2ºC with a relative humidity of 55.5%. A 12-hour light-dark cycle is maintained with automatic timers. All the environmental factors are moderated by a computerized central monitoring system. The animal facility is registered under CPCSEA, Ministry of Environment & Forest, Government of India. The registration number our animal facility is 20/GO/RBi/S/99/CPCSEA for Research, Breeding for in-house use on small animals
The main objective of the Animal House is to supply defined strains of mice, rats, guinea pigs, hamsters and rabbits to the scientific community of the Centre. The propagation of all defined strains is done in two-tier system, i.e., foundation colony (SPF colony) and production colony.
Mouse genotype laboratory was recently established with equipment related to genetic monitoring and biochemical monitoring of laboratory animals. Routine check for genetic contamination in lab animals is carried out using nine microsatellite SSLP markers (D1 Mit 21, D2Mit61,D4 Mit 53, D5 Mit 18, D6Mit39,D9Mit27 ,D12Mit5, and D14Mit7. and seven biochemical markers ( Hbb, Car- I, Gpi- I, Est- I, Alb-1Mup-1 and Trf- I ) and ten biochemical markers ( Hbb, Car- I, Car- II, Gpi- I, Est- I, Est- III, Mod- I, Idh- I, Pgm- I and Trf- I ) using PCR and enzyme markers analyzed through cellulose acetate electrophoresis techniques.
The Animal House team comprises of 18 staff including 11 qualified animal caretakers, who have undergone specialized training in breeding and management of laboratory animals course offered by NCLAS , National Institute of Nutrition, Hyderabad, ACTREC, Mumbai and CSIR-CFTRI, Mysore.
Two trained veterinarian carries out regular health monitoring of all animals to ensure that they are free from contagious diseases. To prevent the transmission of infection, standard hygienic procedures, i.e., use of sterilized bedding, autoclaved cages, zero bacteria/autoclaved drinking water and handling of animals using gloved hands are followed. Necropsy and autopsy of suspected contagious/infectious animals are performed by a veterinarian and prophylactic measures are implemented.
Regular screening of microorganisms in the SPF and production colony mice, feed, water, paddy husk, and isolators are carried out by various microbiological and pathological techniques, viz., culture, differential staining techniques, biochemical tests, ELISA and PCR based microbial screening and histopathology.
The following pathogen were monitored regularly by and ELISA and PCR based monitoring system
Mouse hepatitis virus, Mouse Parvo virus, Mouse Hanta virus, Minute virus of mice, Lymphocytic choriomenigitis, Mouse helicobacter hepaticus, Mouse mycoplasma pulmonis, Rat mycoplasma pulmonis, Kilham rat virus, Rat Corono virus,RCV/SDA and Rabbit pasturella multocida.
Animal house activities are maintained in three different kind of software operated in single platform called as ONTEXA. These includes online animal indenting platform in which PI can raise the online animal request as per IAEC project and once the animal issued, the software automatically create a form D as per CPCSEA requirement. The second platform operated with the inventory software to handle the animal census, mortality, animal production and supply data’s and these data’s are converted to Form C as per CPCSEA requirement. Third platform create a monitoring software to gather data’s and made a report of temperature , humidity monitoring details , fumigation, genetic monitoring and microbial monitoring details
List of Animals are available at CCMB Animal House
S.No |
Strain Name |
Human disease model |
Source |
1 |
B6.129S4-Icam1tm1Jcgr/J
https://www.jax.org/strain/002867 |
Cardiovascular disorder |
Jackson Laboratory, USA |
2. |
B6.129P2-Apoetm1Unc/J
https://www.jax.org/strain/002052 |
Cardiovascular and neurological disorders |
Jackson Laboratory, USA |
3 |
MMTV Cre (Mouse mammary tumour virus)
https://www.jax.org/strain/003553 |
useful for creation of conditional BRCA1 knockout mice- breast cancer |
National cancer Institute, USA |
4 |
B6.129S2-Trp53tm1Tyj/J
https://www.jax.org/strain/002101 |
Cancer research |
Jackson Laboratory |
5 |
NOD/LtJ
https://www.jax.org/strain/001976 |
Type I diabetes insulin-dependent diabetes mellitus |
Jackson Laboratory, USA |
6 |
B6.BKS-Leprdb (Leprdb/Leprdb)
https://www.jax.org/strain/000697 |
Type I diabetes |
Jackson Laboratory, USA |
7 |
B6C3Tg(APPswee PSEN1dE9)85 Dbo/J
https://www.jax.org/strain/004462 |
Alzheimer’s disease model |
Jackson Laboratory, USA |
8 |
Pitx3ak/2 J
https://www.jax.org/strain/000942 |
Parkinson disease model |
Jackson Laboratory, USA |
9 |
APC Cre/ APC flx |
Colon cancer |
Cardiff University, UK |
10 |
BAP (BRCA 1 -/ - / p53 -/- ) |
Breast Cancer |
Cardiff University, UK |
11 |
CD-1 FOX n1nude |
Immunodeficient mice( Deficient in T lymphocytes ) |
Charles River, USA |
12 |
NOD. CB17-Prkdc scid/J https://www.jax.org/strain/001303
|
Deficiency of B & T lymphocytes (severe combined immuno deficient mice) |
Jackson Laboratory, USA |
13 |
Balb C/J
https://www.jax.org/strain/000651 |
Inbred mice |
Jackson Laboratory |
14 |
CBA/ca J
https://www.jax.org/strain/000654 |
Inbred mice |
Jackson Laboratory |
15 |
129 Xl/ SvJ
https://www.jax.org/strain/000691 |
Inbred mice |
Jackson Laboratory |
16 |
Neo r |
Neomycin resistance |
National Institute of Immunology, Delhi |
17 |
Y del |
Inbred mice |
Jackson Laboratory, USA |
18 |
C57BL 6/J
https://www.jax.org/strain/000664 |
Inbred mice |
Jackson Laboratory, USA |
19 |
C57BL 6 /N crl
https://www.jax.org/strain/000664 |
Inbred mice |
Charles River, USA |
20 |
CD-1 |
Out bred mice |
Charles River, USA |
21 |
129-Alp1<tm1(cre)Nagy.
https://www.jax.org/strain/008569 |
Inbred mice |
Jackson Laboratory, USA |
22 |
Dicer 1< tm1Bdh>
https://www.jax.org/strain/006001 |
Inbred mice |
Jackson Laboratory, USA |
23 |
B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ
https://www.jax.org/strain/009086 |
Targeted Mutation |
USA |
24 |
B6;129-Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa/Mmjax
https://www.jax.org/strain/004807 |
Alzheimer's disease |
MMRRC, USA |
25 |
B6.Cg-Tg(SOD1*G37R) 29Dpr/J
https://www.jax.org/strain/008229 |
Amyotrophic Lateral Sclerosis (ALS) |
Jackson Lab,USA |
26 |
C57BL/6J-Tg(Th-SNCA*A30P*A53T)39Eric/J
https://www.jax.org/strain/008239 |
Parkinson Disease |
Jackson Lab, USA |
27 |
FVB/N- Tg(HTT*97Q)IXwy/J
https://www.jax.org/strain/008197 |
Huntington’s disease |
Jackson Lab, USA |
28 |
Tg(Chx10-EGFP/Cre,ALPP)2Clc/J https://www.jax.org/strain/005105 |
Retinoblastoma |
St Jude Children’s Research Hospital,USA/ Jackson Lab, USA |
29 |
B6;129S4-Col/1a1(tetO-Pou5f1-Klf4-Sox2-Myc)Hoch>/J
https://www.jax.org/strain/011001 |
Cancer,Cell biology and genetic research |
Jackson Lab, USA |
30 |
B6.Cg-Gt(ROSA)26Sor<tm1(rtTA*M2)Jae>/J
https://www.jax.org/strain/006965 |
Neurobiology Research |
Jackson Lab, USA |
31 |
B6.129p2(Cg)-Zbtb7b<tm2litt>/J
https://www.jax.org/strain/027663 |
Immunology, Inflammation and Autoimmunity Research |
Jackson Lab, USA |
32 |
B6129SF2/J
https://www.jax.org/strain/101045 |
Inbred mice |
Jackson Lab, USA |
33 |
Tg(DR4)1Jae/J
https://www.jax.org/strain/003208 |
multiple-drug resistant mouse embryonic fibroblasts |
Jackson Lab, USA |
34 |
TgPaxTngFp |
GPF induced |
NCBS Banglore |
35 |
FVB/Nj |
Inbred mice |
ACTREC Mumbai |
36 |
CD-1 FOX n1nude |
Immunodeficient mice( Deficient in T lymphocytes |
Jackson Lab, USA |
Other animals
S.No |
Strain Name |
Human disease model |
Source |
1 |
Wistar Rats |
Out bred |
Charles River laboratories,USA |
2 |
Sprague Dawley Rats |
Out bred |
Charles River laboratories, USA |
3
|
Newzeland white Rabbits |
Out bred |
National Institute of Nutrition, Hyderabad |
4 |
Golden Syrian Hamster |
Out bred |
National Institute of Nutrition, Hyderabad |
|